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two piece guide rna  (Integrated DNA Technologies)


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    Integrated DNA Technologies two piece guide rna
    Two Piece Guide Rna, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 96/100, based on 1012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+rna+oligo/bio_rxiv__2023__01__18__524550-47-18-25?v=Integrated+DNA+Technologies
    Average 96 stars, based on 1012 article reviews
    two piece guide rna - by Bioz Stars, 2026-08
    96/100 stars

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    <t>Insertion</t> of guide <t>RNA</t> oligos into PX458 and PX459 vectors. (A) Oligos for single guide RNA (sgRNA) targeting TP53 and SMARCB1 genes were inserted separately into the BbsI regions of two different vectors, PX458 (GFP) and PX459 (Puromycin resistance), to construct a total of 4 distinct plasmids. (B) These plasmids were checked for the presence of the sgRNA-coding sequences that targets (i) TP53 and (ii) SMARCB1 . The green highlighted sequences are the inserts for sgRNA.
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    Insertion of guide RNA oligos into PX458 and PX459 vectors. (A) Oligos for single guide RNA (sgRNA) targeting TP53 and SMARCB1 genes were inserted separately into the BbsI regions of two different vectors, PX458 (GFP) and PX459 (Puromycin resistance), to construct a total of 4 distinct plasmids. (B) These plasmids were checked for the presence of the sgRNA-coding sequences that targets (i) TP53 and (ii) SMARCB1 . The green highlighted sequences are the inserts for sgRNA.

    Journal: Bioactive Materials

    Article Title: Modeling human brain rhabdoid tumor by inactivating tumor suppressor genes in induced pluripotent stem cells

    doi: 10.1016/j.bioactmat.2023.08.009

    Figure Lengend Snippet: Insertion of guide RNA oligos into PX458 and PX459 vectors. (A) Oligos for single guide RNA (sgRNA) targeting TP53 and SMARCB1 genes were inserted separately into the BbsI regions of two different vectors, PX458 (GFP) and PX459 (Puromycin resistance), to construct a total of 4 distinct plasmids. (B) These plasmids were checked for the presence of the sgRNA-coding sequences that targets (i) TP53 and (ii) SMARCB1 . The green highlighted sequences are the inserts for sgRNA.

    Article Snippet: Insertion of guide RNA oligos into PX458 and PX459 vectors. (A) Oligos for single guide RNA (sgRNA) targeting TP53 and SMARCB1 genes were inserted separately into the BbsI regions of two different vectors, PX458 (GFP) and PX459 (Puromycin resistance), to construct a total of 4 distinct plasmids. (B) These plasmids were checked for the presence of the sgRNA-coding sequences that targets (i) TP53 and (ii) SMARCB1 .

    Techniques: Construct